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Substrate solution is then added and color develops in proportion to the amount of Human EAF2
The assay plate has been pre-coated with mouse anti-Human TNF beta monoclonal antibody
The plate has been pre-coated with Human ACTN4 antibody
The plate has been pre-coated with Rat GDF11 antibody
Rat NT-3 (Neurotrophin 3) ELISA Kit AQP4 Substrate solution is then addedThe Rat (NT 3) Neurotrophin 3 ELISA Kit measures Neurotrophin 3 in Rat samples. The plate has been pre coated with Rat NT 3 antibody. NT 3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat NT 3 Antibody is added and binds to NT 3 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated NT 3 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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